What we work on
Functions of the nuclear lamina
We explore how the nuclear lamina scaffolds chromatin organization and sustains nuclear integrity in both pluripotent and differentiating cells. We manipulate the lamina using genetic perturbations and acute degron depletion tools. We apply genomics assays and live-cell microscopy to probe the functions of the lamina.
Proteostasis at the nuclear lamina
We use metabolic labeling and dynamic proteomics to profile nuclear protein homeostasis. We discovered that the nuclear lamina is an extremely long-lived protein structure but undergoes turnover in some disease states and developmental contexts. We are working to understand lamina biogenesis, quality control, and maintenance over lifespan.
The lamina and human disease
Hundreds of mutations to the lamina and lamina-associated proteins cause “laminopathy” syndromes. We apply deep mutational scanning and proteomic methods to dissect the consequences of disease mutations on lamina assembly, structure, and function in order to enable informed decisions about treatment of these devastating syndromes.
